Role of LpnA in Francisella tularensis Susceptibility to Resazurin.
نویسندگان
چکیده
Francisella tularensis is defined by the Centers for Disease Control and Prevention as a Category A bioterrorism agent due to its low infectious dose, high mortality rate, ease of aerosolization. Given there no licensed tularemia vaccine in United States possible development release antibiotic-resistant F. strains, an urgent need new treatments against this bacterium. We determined phenoxazine dye resazurin (Rz) exhibits antimicrobial activity other gram-negative bacteria. The mode action compound not understood, but potential targets Rz were identified throughput screen resistant isolates. Ninety-three percent Rz-resistant (Rzr) isolates sequenced contained mutations within coding regions FTL_0421 (lpnA), FTL_0895, FTL_1504 (katG). To confirm mutation lpnA was contributing resistance, we introduced wild-type copy into Rzr1 then assessed susceptibility resulting complemented strain (Rzr1/pABST-lpnA). minimum inhibitory concentration (MIC) Rzr1/pABST-lpnA similar, therefore, wanted restoring expression LpnA via Western blot analysis. As expected, expressed LVS genetic mutation. In transformed with pABST-lpnA, still did observe suggesting our complementation strategy ineffective. future, plan take alternative approach investigate role resistance creating deletion mutant.
منابع مشابه
The use of resazurin as a novel antimicrobial agent against Francisella tularensis
The highly infectious and deadly pathogen, Francisella tularensis, is classified by the CDC as a Category A bioterrorism agent. Inhalation of a single bacterium results in an acute pneumonia with a 30-60% mortality rate without treatment. Due to the prevalence of antibiotic resistance, there is a strong need for new types of antibacterial drugs. Resazurin is commonly used to measure bacterial a...
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ژورنال
عنوان ژورنال: Proceedings of the West Virginia Academy of Science
سال: 2023
ISSN: ['0096-4263', '2473-0386']
DOI: https://doi.org/10.55632/pwvas.v95i2.968